Composite

Part:BBa_I2053

Designed by: Bartholomew Canton   Group: iGEM06_MIT   (2007-06-16)

AHL regulated synthesis of T7 RNAP

The AHL receiver device, BBa_F2620, controls the synthesis of T7 RNAP. The downstream terminator is a T7 RNAP terminator, so its unlikely to provide efficient termination of E. coli RNAP. This terminator is being used because it will be part of a later construct using this T7 RNAP protein generator

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3751
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004


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Categories
Parameters
None